Each personal computer or web server is run by an Operating System - the low-level program which interacts with the computer hardware, including peripherals such as a mouse and a printer, together with any apps that are present on your system. Any program input using a command line or a Graphical User Interface (GUI) is processed by sending an Application Program Interface (API) request to the OS. On a server, any application runs within the parameters defined by the OS as well - priority, physical memory, processing time, etc. This goes for both standard website scripts and server-side software like a media server. If a virtual server is generated on a physical one, there can be two different Operating Systems, called guest OS and host OS, so you will be able to set up a different software environment on a single machine.